"Tupi Animal Lore enables you to train Tame Cougars from your Town Center."—In-game description
Tupi Animal Lore is a technology in Age of Empires III that can be researched at a Trading Post built on a Tupi settlement. Once researched, it allows Buttercup the Pet Cougars to be trained at Town Centers.
Tupi Animal Lore is largely useless as while it adds some population free units to the player's attack force, it is hard to work them out into a strategy against flesh and blood opponents.